Composite

Part:BBa_K1648003:Design

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-07-17)


Recombination Template for mamXY,mamGC and mms Operons with Promotor and Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1413
    Illegal BamHI site found at 512
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 683
    Illegal NgoMIV site found at 865
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1363
    Illegal AgeI site found at 566
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 550
    Illegal BsaI site found at 649
    Illegal BsaI site found at 930


Design Notes

In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from mamHIEJKLMN and mamPAQRBSTU (parts of mamAB operon without the mamO operon) as flanking sequences. Fragments are joined via overlapping PCR.

K1648003 history.png

Source

Magnetospirillum gryphiswaldense MSR-1 v2, complete genome

References